Application

Western Blotting Analysis: A representative lot detected the ATP-dependent recruitment of XPA in HeLa nuclear extract onto the stalled RNA pol IIO on mono-cisplatin-damaged DNA (Riedl, T., et al. (2003). EMBO J. 22(19):5293-5303; Laine, J.P., and, Egly, J.M. (2006). EMBO J. 25(2):3873-97).
Immunocytochemistry Analysis: A representative lot detected XPA recruitment to the DNA damage sites in the nuclei of UV-irradated HeLa cells (Alekseev, S., et al. (2014). Chem Biol. 21(3):398-407).

Research Sub CategoryNuclear Receptors

Research CategoryEpigenetics & Nuclear Function

Anti-XPA Antibody, clone 1XPA Antibody-1E11 is an antibody against XPA for use in Western Blotting, Immunocytochemistry.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

DNA repair protein complementing XP-A cells (UniProt P23025; also known as Excision repair-controlling, Mutant xeroderma pigmentosum complementation group A, Xeroderma pigmentosum group A-complementing protein) is encoded by the XPA (also known as XP1, XPAC) gene (Gene ID 7507) in human. DNA lesions caused by UV irradiation, drugs, or other environmental factors are eliminated by two nucleotide excision repair (NER) pathways, Global genome repair (GGR) and transcription-coupled repair (TCR). In GGR, the removal of lesions requires their recognition by the repair factor XPC/HR23b and the subsequent opening of the DNA duplex by transcription factor II human (TFIIH). The resulting single-stranded structure is stabilized by XPA and replication protein A (RPA). XPG is recruited through its interaction with TFIIH on the 3′ side of the lesion and its positioning on the cut site requires RPA. The interaction between XPA and XPB (ERCC1) stimulates the recruitment of ERCC1-XPF on the 5′ side of the DNA lesion. The damaged oligonucleotide can then be removed through the double incision by XPG and ERCC1-XPF endonucleases. In TCR, these factors (except XPC/HR23B) are recruited by the stalled RNA pol II in front of the damage with the help of the CSB and CSA proteins.

Immunogen

Linear peptide corresponding to human XPA sequence near C-terminus.

Epitope: Near C-terminus

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Mouse monoclonal IgG1κ ascites with 0.05% sodium azide.

Unpurified

Quality

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected XPA in 10 µg of HeLa nuclear extract.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~37 kDa observed. Target band appears larger than the calculated molecular weight of 31.4 kDa, most likely due to posttranslational modifications. Uncharacterized band(s) may appear in some lysates.